Possible reason:Enzymatic activity of endogenous peroxides is not completely blocked

Solution:Block with 3% H2O2

Possible reason:Non-specific dyeing

Solution:Add 10% normal goat serum before adding antibody

Possible reason:The antigen is diffused due to autolysis of tissues

Solution:Use fresh tissues or fixed tissues in time as far as possible

Possible reason:Incomplete deparaffinating

Solution:Replace xylene and alcohol

Possible reason:Sections are flushed insufficiently

Solution:Flush completely

Possible reason:Enzyme reaction accelerates at too high room temperature

Solution:Ensure that the room temperature is 15-25℃ to shorten the developing time

Possible reason:Sections are dry during dyeing

Solution:Ensure that sections are moist